Master 2nd year

Semester 3 & Semester 4

BioImaging (BIM)

Chairs

Bruno Dubois, PhD

Institut d'Optique, ParisTech

Karsten Plamann, PhD

ENSTA ParisTech

BIM@bme-paris.org

Teaching Faculty

...

ester 4

Course Schedule:
Lecture 1: Non-linear microscopy / Super-resolution imaging: Two-photon excitation fluorescence microscopy, Harmonic generation microscopy , Coherent Anti-Stokes Raman Scattering (CARS) microscopy.Total Internal Reflection Fluorescence microscopy (TIRF), 4π microscopy, Stimulated Emission Depletion microscopy (STED), Stochastic Optical Reconstruction Microscopy (STORM), PhotoActivated Localization Microscopy (PALM). (Ecole Polytechnique)
Lecture 2: Optical Coherence Tomography: Time-domain OCT, Frequency-domain OCT, Full-field OCT, Extensions (polarization-sensitive, doopler, spectroscopic), Applications in biology and medicine. (Institut d'Optique)
Lecture 3: Optical tweezers: The physics of optical tweezers, technical issues, characteristics of optical tweezers, application in microbiology and single molecule manipulation. (Institut d'Optique)
Lecture 4: Light propagation in tissues: Light scattering by small particles, scattering cross sections, orders of magnitude, multiple scattering, ballistic and diffuse intensity, mean free paths, time and length scales, modelling light transport in tissues, diffusion approximation. (ESPCI ParisTech)
Lecture 5: Holographic microscopy / Optics of ocular tissues: Historical introduction, the wavefront reconstruction problem, Gabor and Leith-Upatnieks holograms, image location and magnification, digital holography Anatomy of the anterior segment of the eye, transparency and light scattering of corneal tissue: initial work by David Maurice, transparency and microstructure, consequences for laser surgery, transparency of the crystalline lens. (ENSTA ParisTech)

Evaluation: written exam (100%)

n the basic sciences (mathematics, physics, chemistry) and applied mathematics (signal-processing, numerical analysis).