Master 2nd year

Semester 3 & Semester 4

BioImaging (BIM)

Chairs

Bruno Dubois, PhD

Institut d'Optique, ParisTech


Pierre Bourdoncle, PhD

Univ. Paris Descartes



BIM@bme-paris.org



Teaching Faculty

- Pierre Bourdoncle, Univ. Paris Descartes
- Arnaud Dubois - H. Benisty, Institut d’Optique Graduate School- ParisTech.
- A. Alexandru-, Ecole Polytechnique ParisTech

...

ester 4

UE 3.2 Optical Imaging level 1

Outline: Introductory courses to optical imaging, microscopy, microarrays. Labworks.

 

 

Course Schedule:
Lecture 1: Introduction to optical imaging: Propagation of light in biological tissues, High-penetration imaging methods (diffuse optical tomography, opto-acoustic imaging), High-resolution imaging methods (microscopy, OCT).
Lecture 2: Optical microscopy:  Conventional microscopy, Confocal microscopy, Nonlinear microscopy (multi-photon, 

harmonic generation).
Lecture 3: Fluorescence techniques: Organic/inorganic fluorophores, Single-molecule tracking, Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Correlation and Cross-Correlation (FCS, FCCS), Fluorescence lifetime imaging (FLIM), Fluorescence Resonant Energy Transfer (FRET).
Lecture 4: DNA and protein microarrays: Readout techniques: fluorescence and Surface Plasmon Resonance, Biochip specifications and realizations, Data processing and interpretation.

Lecture 5-9: Optics Labworks. Various microscopy techniques including: confocal, fluorescence, two-photon excitation fluorescence, second-harmonic generation.


Evaluation: written exam (100%)

n the basic sciences (mathematics, physics, chemistry) and applied mathematics (signal-processing, numerical analysis).